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Observing a specimen under a microscope needs undeniable matriculation. Moreover, if you must look through the microscope for long, the strain on the eye gets unbearable.
Whenever you make changes to the objective lens, the image goes out of focus? That gets quite frustrating while working on the microscope for long periods.
There is a sure and robust solution to that problem as well. The Parfocal lens. No matter how you tinker with the microscope’s objective, your image will have a constant sharp focus.
In this section, we will throw some light on the workings of a parfocal lens. Follow along for some insights into making the most out of such lenses.
In brevity, parfocal objectives require negligible refocusing to be altered. You can easily change objectives with alternating powers.
No matter what changes you bring to the objective lens, the image remains in sharp focus. For this, you must parfocal your objectives.
Parfocalling is a straightforward concept. When your objectives are parfocalled if you switch from one magnification to the other. The objective lens will always remain at a relative focus.
In case when you haven’t parafocalled your objective, it will need constant refocusing. This will occur every time you try to change the magnification.
The absence of parfocalling makes the endeavor inconvenient. It increases eye strain and causes wear and tear of your microscope.
The method of parafocalling varies with different manufacturers.
How do you tell if your microscope is parfocalled or not?
We have cleared the concept of parfocalling. But how do you determine if your microscope is parfocalled or not?
You need to bring in a slide into focus, under settings of highest magnification. Then you must switch to a lower magnification.
This will help you to check how sharp the focus is on the slide. If your slide remains focused despite the little adjustments. The objective is parfocalled.
Whereas, if the slide goes out of focus, the objective is not parfocalled. You must begin with a high-powered objective because of a narrower focus.
How do I parfocal my microscope’s objective lenses?
If your slide goes out of focus consistently, then your objective is not parfocalled. Fret not, as always; we have a solution for you.
Follow along to learn about parfocalling your microscope’s objective. Begin by removing the objective lens that you wish to parfocal.
The objective needs to detach from the microscope. It would help if you then opened the outer covers surrounding the lens.
For objectives of 4x or 10x calibration, leave them be. These objective lenses cannot be adjusted.
You need to use the 10x objective as a base to parfocal the high-powered lens. The adjustments are made in an increasing order beginning for 10x.
1. Removing the outer cover of the objective.
You need to unscrew the cove, to detach the lens. If you remove the cover of multiple covers simultaneously, make sure you don’t mix them up.
2. Adjusting the Parfocal ring.
Begin by turning the parfocality ring. If the ring moves, you are ready to move to the next step.
If the ring does not turn, there must be a cementing material. This is to keep the ring in place to prevent it from rotating.
You need to look for the piece of cement on the ring. Once you have located the cement, a little nudge will remove it from the ring.
3. Testing the objective lens.
After removing the ring from the objective, reinstall it on the microscope. Make sure you install the lenses in ascending order, starting from the 10x lens.
You can use a stage micrometre to focus with the 10x objective. Once that is done, move to the next high-powered objective.
While looking through the objective, don’t use the knobs to focus. Use the adjustment ring on the objective.
Since the adjustment is delicate, ensure your movements are rather slow. Repeat the same process for the other objectives as well.
4. Securing the Parfocal ring.
Now, place a tiny drop of optical cement on the ring. This will help secure the ring in place after you have adjusted.
Using optical cement is not mandatory. We recommend using it, as it keeps the parfocal settings secured.
You can use a drop of nail paint if you don’t have an optical cement. You need to add the cement when the objective is still attached to the nosepiece.
Make sure you give some time for the optical cement to dry. After that, you need to place the screw and the objective cover back into the microscope’s objective.
Parfocalling an objective lens is a one-time endeavor. Once you have completed the steps mentioned above, your microscope will remain parfocalled for a long time.
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